Composite

Part:BBa_K646003

Designed by: David Dubow   Group: iGEM11_Macquarie_Australia   (2011-09-29)

T7 promoter with Hemeoxygenase

Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI.

Successful expression should result in green colonies due to the presence of biliverdin.

Usage and Biology

MQ New data page diagram.jpg

The BioBrick part in team Macquarie University (2011) mechanism

Results - green colonies.JPG

Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green due to the production of biliverdin from the breakdown of heme - this indicates that heme oxyganse was expressed using T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None